Basic fibroblast growth factor promotes doxorubicin resistance in chondrosarcoma cells by affecting XRCC5 expression

Chondrosarcoma is the second most common form of bone cancer and is characterized by its ability to produce an extracellular matrix of the cartilage. High-grade chondrosarcoma is highly aggressive and can metastasize to other parts of the body. Chondrosarcoma is resistant to both conventional chemotherapy and radiotherapy; hence, the current main treatment is still surgical resection. Doxorubicin (Dox) has been shown to significantly improve patient survival compared with untreated chondrosarcoma. However, for patients with metastasis, surgical resection alone can hardly treat them. In addition, drug resistance is one of the leading causes of death in patients with chondrosarcoma. Secreted proteins can mediate cell-cell interactions in the cancer microenvironment, which may be associated with the development of drug resistance. In the present study, chondrosarcoma cells were treated with Dox, the conditioned medium was then collected and changes in secreted proteins were analyzed using the antibody array. Results showed that the Dox-treated group had the highest secretion of basic fibroblast growth factor (bFGF), indicating the effect of bFGF on Dox sensitivity in chondrosarcoma. Furthermore, lentiviral-mediated knockdown and treatment of exogenous recombinant protein were employed to further investigate the effect of bFGF on Dox resistance. Results demonstrated that bFGF can promote the expression of X-ray repair cross-complementing protein 5 (XRCC5), leading to Dox resistance. Secreted bFGF is likely to be detected in serum, in addition to being a biomarker for predicting Dox resistance, the combination of Dox and bFGF/XRCC5 blockers may be a new therapeutic strategy to improve the efficacy of Dox in future.     

风池穴不同刺法对椎动脉型颈椎病的临床疗效评价

目的:探究风池穴不同刺法对椎动脉型颈椎病的临床疗效。方法:选择2018年4月—2019年1月北京中医药大学东直门医院针灸科门诊就诊的84例椎动脉型颈椎病患者作为研究对象,分为观察组43例、对照组41例。观察组采用短刺法针刺风池穴治疗,对照组采用平补平泻法针刺风池穴治疗,1个疗程后比较两组患者的ESCV、颈椎病症状分级量化表评分以及TCD相关指标,TCD指标观察基底动脉和双侧椎动脉的平均血流速度(Vm)、收缩期血流速度峰值(Vs)、血管搏动指数(PI)和血管阻力指数(RI)的变化。结果:治疗前后两组ESCV、颈椎病症状分级量化表评分比较,差异有统计学意义(P<0.05),且椎动脉、基底动脉的Vs、Vm较治疗前明显减慢,且观察组治疗效果优于对照组(P<0.05)。结论:针刺风池穴治疗椎动脉型颈椎病临床效果显著,且短刺法优于平补平泻法。     

Risk factors of rib metastases in nasopharyngeal carcinoma patients with rib 99mTc- MDP foci on whole-body bone scansCSCD

Objective To investigate the correlation of rib 99mTc-MDP foci on whole-body bone scan with clinical variables and rib metastases in nasopharyngeal carcinoma(NPC) patients, and to screen the risk factors of rib metastases. Methods We retrospectively reviewed 312 NPC patients with rib 99mTc-MDP foci on wholebody bone scan. Chi-square test and logistic regression were performed to evaluate the correlation between clinical variables and rib metastases. Results In all 312 NPC patients, rib metastases were associated with T stage, skull base bone invasion, other bone metastasis, number of rib foci, lateral localization on rib and foci type (P<0.01), and the risk factors of rib metastasis included skull base bone invasion, other bone metastases, lateral localization on rib and foci type (P<0.05). In 176 patients with pure rib foci, rib metastases were closely related to T stage, skull base bone invasion, other bone metastasis, number of rib foci and lateral localization on rib (P<0.05), while only lobar distribution (P=0.029) was the effective risk factor. In 198 patients with single rib focus, rib metastases were affected by skull base bone invasion and foci type (P<0.01), while only foci type (P=0.000) was the effective risk factor. In all 566 rib foci, uptake level and localization on rib were the effective risk factors of rib metastases(P<0.01). Conclusion In NPC patients with rib foci on whole body bone scan, the effective risk factors of rib metastases include skull base bone invasion, other bone metastases, lateral localization on rib, foci type, uptake level and anterior and posterior localization on rib. Follow up should be the main way for the pure rib foci on unilateral ribs. For multiples rib foci on bilateral ribs or single rib focus combined with other bones foci, additional image modalities should be required to exclude bone metastasis. © 2020, CHINA RESEARCH ON PREVENTION AND TREATMENT. All rights reserved.     

丹参注射液对带蒂皮瓣移植修复术后成活率及预后的影响

目的:探讨丹参注射液对带蒂皮瓣移植修复术后成活率及预后的影响。方法:将68例接受带蒂皮瓣移植修复术的患者简单随机分成观察组与对照组各34例。对照组给予术后常规治疗,观察组在此基础上加用丹参注射液治疗,持续给药1周。随访3～12个月,比较两组患者术后皮瓣成活率、皮瓣肿胀程度、凝血指标凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(FIB)水平变化及并发症发生率。结果:观察组患者皮瓣成活率为97.06%,明显高于对照组的76.47%,差异有统计学意义(P<0.05)。观察组术后7 d皮瓣肿胀程度明显轻于对照组,差异有统计学意义(P<0.05)。观察组患者术后3 d的PT、APTT、FIB水平[(17.84±2.45)s、(35.38±3.14)s、(2.24±0.17)g/L]均明显低于对照组患者[(23.07±2.69)s、(41.56±3.34)s、(2.79±0.32)g/L],差异有统计学意义(P<0.05)。观察组患者术后伤口感染、瓣下积液、动静脉危象的总并发症率为8.82%,明显低于对照组的29.41%,差异有统计学意义(P<0.05)。结论:丹参注射液能提高带蒂皮瓣移植修复术后成活率,减轻术后皮瓣肿胀程度,改善凝血指标,降低血管危象、皮瓣坏死等并发症风险。     

活血利水法在骨伤科疾病中的应用

基于骨伤疾病瘀血内阻的重要病机和现代医家"治血重治水"的学术观点,探索概括了活血利水法在骨伤疾病中的应用现状,结合现代医学的认识,全方位分析了活血利水法的优势和缺点。活血利水法可应用于骨折、筋伤等骨伤科大部分疾病及骨伤科疾病的围手术期,能改善肢体肿胀,促进伤口愈合,便于患者早期功能康复,但目前的临床应用尚缺乏统一的规范和标准,且处方不良反应尚未完全阐明。     

北京地区3859名体检人群骨密度调查及骨量异常患病率分析

目的调查北京地区健康体检人群骨密度的情况以及骨量减少和骨质疏松的患病率,为骨质疏松症的防治提供参考。方法选择2017年1月至2018年12月在中日友好医院健康体检中心进行健康体检的人群,排除继发性骨质疏松症及其他影响骨代谢的因素,共3859名。其中男性2067名,女性1792名。年龄20~83岁,平均年龄(51.29±11.18)岁,按性别及年龄每10年一组。采用美国GE公司的LUNAR Prodigy双能X线骨密度仪测量受试者腰椎1~4正位及股骨颈和全髋的骨密度。分析各组不同部位骨密度情况及骨量异常(包括骨量减少和骨质疏松)的患病率。采用SPSS 22.0统计软件进行分析,以P<0.05为差异有统计学意义。结果①男性腰椎1~4骨密度峰值在20~29岁,股骨颈和全髋骨密度峰值在30~39岁。女性各部位骨密度峰值均在30~39岁。②随年龄增长,男性和女性骨量异常患病率均呈上升趋势,50岁以上女性骨量异常患病率显著上升,明显高于同年龄组男性。③30~59岁男性和女性腰椎骨量异常患病率均明显高于髋部;70岁以上男性和60岁以上女性髋部骨量异常患病率明显高于腰椎。结论中老年人群尤其是绝经后女性是骨质疏松症的高危人群;老年人群的骨质疏松筛查可以考虑选择髋部骨密度为主。     

针刀松解寰枕筋膜治疗颈源性眩晕的疗效研究

目的:研究针刀松解寰枕筋膜治疗颈源性眩晕的疗效。方法:选取2017年1月至2018年1月佛山市健翔医院收治的颈源性眩晕患者96例作为研究对象,按照随机数字表法随机分为对照组和观察组,每组48例。对照组患者给予3次/周针灸、局部按摩治疗,观察组患者给予1次/周的针刀松解寰枕筋膜治疗,均治疗3周。依照《颈性眩晕评估量表(ESCV)》分析2组患者治疗前后颈性眩晕评分变化、椎动脉血流动力学变化,比较2组患者血清中内皮源性超极化因子(EDHF)的含量和一氧化氮(NO)水平,统计2组患者的治疗有效率、眩晕症状缓解时间及生命质量变化。结果:观察组患者治疗后总有效率为97.92%高于对照组的81.25%(P<0.05),眩晕有效缓解时间明显缩短,眩晕评分显著高于对照组(P<0.05);观察组患者治疗后椎动脉的平均血流、收缩期血流、舒张期血流速度、血清中NO及EDHF水平明显上升,血管搏动指数显著下降(P<0.05);生命质量评分显著升高(P<0.05)。结论:针刀松解寰枕筋膜能够通过改善颈源性眩晕患者的椎动脉血流动力学,有效治疗患者的颈源性眩晕。     

滋肾续骨汤联合鲑鱼降钙素对老年椎体压缩性骨折术后血清骨形态发生蛋白9及血管内皮生长因子水平的影响

目的观察滋肾续骨汤联合鲑鱼降钙素对老年椎体压缩性骨折术后血清骨形态发生蛋白9(BMP9)及血管内皮生长因子(VEGF)水平的影响。方法将80例老年椎体压缩性骨折术后患者按照随机数字表法分为2组。对照组39例予鲑鱼降钙素治疗;治疗组41例在对照组治疗基础上加用滋肾续骨汤治疗。2组均治疗24周后统计临床疗效,并观察2组治疗前后血清BMP9、VEGF水平及疼痛视觉模拟评分(VAS)、胸腰椎损伤分类及损伤程度评分系统(TLICS)、Oswestry功能障碍指数(ODI)评分、日本骨科协会(JOA)腰痛评分,以及椎体前缘丢失高度、伤椎Cobb角、伤椎骨密度水平及并发症发生情况。结果治疗组总有效率95.12%(39/41),对照组总有效率76.92%(30/39),治疗组临床疗效优于对照组(P<0.05)。2组治疗后血清BMP9、VEGF水平均升高(P<0.05),且治疗组高于对照组(P<0.05)。2组治疗后疼痛VAS、TLICS评分均降低(P<0.05),且治疗组低于对照组(P<0.05)。治疗后2组ODI评分均降低(P<0.05),且治疗组低于对照组(P<0.05);治疗后2组JOA腰痛评分均升高(P<0.05),且治疗组高于对照组(P<0.05)。治疗后2组椎体前缘丢失高度、伤椎Cobb角均降低(P<0.05),伤椎骨密度均升高(P<0.05),且治疗组改善均优于对照组(P<0.05)。治疗组并发症总发生率4.88%(2/41),对照组并发症总发生率20.51%(8/39),治疗组并发症总发生率低于对照组(P<0.05)。结论滋肾续骨汤治疗老年椎体压缩性骨折术后患者疗效显著,可有效改善临床症状及血清BMP9、VEGF水平。     

维生素B12和叶酸干预对绝经后骨质疏松症妇女的骨代谢及同型半胱氨酸水平的影响

目的观察叶酸和维生素B12对绝经后骨质疏松症妇女的骨代谢及同型半胱氨酸水平的影响。方法 80例绝经后骨质疏松症妇女参加研究。所有参与者随机接受叶酸和维生素B12(n=40)或安慰剂(n=40)治疗。在干预前的基线及干预后3个月和6个月,测量两组患者血清同型半胱氨酸、维生素B12和骨代谢标志物的水平。结果治疗前,两组患者血清同型半胱氨酸、维生素B12和骨代谢标志物水平比较差异无统计学意义(P0.05)。治疗后,两组患者同型半胱氨酸均下降,但比较差异无统计学意义(P0.05)。6个月后各组间血清维生素B12、骨钙素、CTX的变化均有显著改变且差异有统计学意义(P0.05)。结论绝经后骨质疏松症妇女补充叶酸和维生素B12可以一定程度改善同型半胱氨酸及骨代谢指标水平。